The UNGC currently occupies approximately 1500s sq/ft of dedicated laboratory space in the Gonda research facility on the UCLA campus. Installed capital equipment includes one PacBio Sequel IIe long read sequencer and access to NovaSeq 6000 and NovaSeq X plus instruments, one Illumina LIMS capable iScan confocal laser scanner with Autoloader II automatic loading support capable of scanning all Illumina beadchip formats. One Tecan Evo 150 robotic liquid handling platform with Illumina GTS and Infinium robot control software installed, one Tecan Evo 100 robotic liquid handling platform with disposable tip Air LiHa. One Tecan Fluent 780 liquid handler with disposable tip Air LiHa and two 48 place temperature controlled beadchip processing racks, one SciGene Little Dipper microchip processing robot, one Tomtec autosealer, one MJ Research tetrad 2 thermocycling system and four Eppendorf Nexus gradient PCR machines and one QuantStudio 3 RT-PCR machine. Additional equipment includes one Covaris M220 nucleic acid shearing system, one Covaris R230 high throughput nucleic acid shearing platform one VisonMate SR 2D barcode plate scanner, one Agilent 4200 Tapestation, one Caplier Labchip XT nucleic acid size selection system, six programmable incubation ovens, six microplate heat blocks, two tabletop centrifuges,one Molecular Dynamics fluorescent microplate readers, one speedvac and four high capacity microplate shakers, 8 benchtop precision shakers and -20 and -80 freezer storage. Computer resources include ten networked workstations. The UNGC has 60TB of network storage space with RAID backup.
The UNGC is equipped to provide sequencing services, including library preparation and QC, using all current Illumina and compatible third party chemistries and kits.
The UNGC supports all versions of Illumina’s whole genome and custom iSelect Infinium genotyping assays, including methylation analysis.
The UNGC is directed by Dr. Nelson Freimer. The UNGC is committed to providing equivalent access to its members at all institutions.
New consortium members are always welcome, contact Nelson Freimer for details at nfreimer@mednet.ucla.edu or 310-794-9571. Cost estimates and information regarding all core services may be obtained by contacting Joe DeYoung, the facility manager at: jdeyoung@mednet.ucla.edu or by phone at 310-825-2390
Additional information can be found on our web site: http://www.semel.ucla.edu/ungc